·
搜索结果:找到“Stanley Caveney”相关结果9条
排序: 按相关 按相关 按时间降序
  • 【专利】 Insect monoamine transporter and methods of use thereof

    作者:Tabita Malutan ; Cameron Donly ; Stanley Caveney 年份:2004
    摘要:The present invention is directed to a lepidopteran octopamine/tyramine transporter. Nucleic acids encoding the transporter, and vectors and host cells comprising the nucleic acids are provided. The invention further provides a recombinant octopamine/tyramine transporter, kits and compositions comprising the transporter, and methods of identifying agents that mediate the activity of the transporter.
  • 【期刊】 New Observations on the Secondary Chemistry of World Ephedra (Ephedraceae)

    刊名:American Journal of Botany 作者:Stanley Caveney ; David A. Charlet ; Helmut Freitag ; Maria Maier-Stolte and Alvin N. Starratt 年份:2001
    摘要:http://www.jstor.org/stable/3558330
  • 【期刊】 Patterns of Daily Flight Activity in Onitine Dung Beetles (Scarabaeinae: Onitini)

    刊名:Oecologia 作者:Stanley Caveney ; Clarke H. Scholtz and Peter McIntyre 年份:1995
    摘要:http://www.jstor.org/stable/4221058
  • 【期刊】 Patterns of daily flight activity in onitine dung beetles (Scarabaeinae: Onitini)

    刊名:Oecologia (Berlin) 作者:Stanley Caveney ; Clarke H. Scholtz ; Peter McIntyre 年份:1995
    摘要:Different species of African dung beetles emerge from the soil at characteristic times of the day to fly and colonize the freshly-deposited dung of mammalian herbivores. Onitine dung beetles in their natural habitat displayed one of five distinctive daily flight behaviours: dusk crepuscular (Onitis alexis Klug, O. caffer Boheman, O. fulgidus Klug, O. tortuosus Houston, O. vanderkelleni Lansberge, O. westermanni Lansberge); dusk/dawn crepuscular (O. pecuarius Lansberge and O. viridulus Boheman); dusk/dawn crepuscular and nocturnal (O. aygulus (Fabricius), O. mendax Gillet, O. uncinatus Klug); late afternoon-dusk and dawn-early morning (Heteronitis castelnaui (Harold)); or diurnal flight activity (O. belial (Fabricius), O. ion (Olivier)). These diagnostic daily flight behaviours span a light intensity range of over 6 orders of magnitude and have been retained in selected species introduced into Australia. Ambient light intensity appears to be the primary determinant of the daily flight period in onitine dung beetles. Because the dung of mobile herbivores is rapidly exploited by onitine species for feeding and breeding purposes, different flight behaviours result in a spatial and temporal partitioning of species in the local dung beetle community. The timing of flight may contribute to, or lead to avoidance of, competition between species which may ultimately affect colonization success. Many onitines show a strong preference for dung of specific herbivores, which may further reduce interspecific competition. All crepuscular-nocturnal species examined raised their thoracic temperatures endothermically to between 35 degree C and 40 degree C before the onset of flight. In O. aygulus the thoracic temperature excess was as large as 19.3 degree C. The thermal threshold below which the frequency of flight onsets drops off rapidly is about 12 degree C for O. aygulus and 17 degree C for O. alexis and O. pecuarius. Radiant loss of body heat during cool nights and dawns may explain why smaller species ( lt 0.4 g body weight), in particular, are adapted behaviourally so that they fly only during the day or early dusk.
  • 【期刊】 A gradient of morphogenetic information involved in muscle patterning.

    刊名:Journal of embryology and experimental morphology 作者:Gareth Williams ; Stanley Caveney 年份:未知
    摘要:The results of grafts performed in the larva of the beetle Tenebrio molitor reveal that the position at which certain adult muscle attachments form is specified by a regulative, rather than a mosaic, mechanism. The results of grafts involving the rotation of squares of integument through 180 degrees or 90 degrees, or the antero-posterior transposition of two adjacent rectangles of integument, show that the site of muscle attachments in the antero-posterior axis of an abdominal sternite is specified by an epidermal segmental gradient of positional information. This gradient is presumed to be identical to the gradient which specifies cuticular patterns in this insect. There is a good correlation between the effect of grafts on adult muscle morphology and cuticular patterns.
  • 【会议】 l-Glutamate retrieved with the moulting fluid is processed by a glutamine synthetase in the pupal midgut of Calpodes ethlius

    作者:C. Yarema ; Heather McLean ; Stanley Caveney 年份:未知
    摘要:From apolysis until pupal ecdysis, the pharate pupa of the Brazilian Skipper (Calpodes ethlius) lies wrapped in a prepupal shell composed of the larval cuticle and an ecdysial space (ES) filled with enzyme-rich moulting fluid (MF). In the 4 h before ecdysis the pharate pupa drinks the moulting fluid through its mouth and anus, and transfers the cuticular degradation products to its midgut (MG). At the same time, extra fluid passes across the body wall of the pharate pupa and flushes out the ES. The MF is recovered at an overall rate of 70 μl/h and reabsorbed across the pharate pupal midgut at about 26 μl/h. l-Glutamate was found to be the dominant amino acid in the moulting fluid. Total MF glutamate peaked at 850 nmol about 8 h before pupal ecdysis (P28), but by ecdysis it had dropped to nearly zero as the MF became diluted with new fluid and was consumed. The drop in glutamate in the ES coincided with a rise in the glutamine content of the fluid in the midgut lumen. The highest rate of glutamine synthesis occurred in midguts isolated from pharate pupae actively drinking MF (P#24). The enzyme glutamine synthetase (GS) was found to be active in glutamate metabolism in the pharate pupal midgut. Glutamine synthesis in the midgut was l-glutamate-dependent and inhibited by two selective competitive inhibitors of GS activity, l-methionine sulfoximine (MSO) and glufosinate ammonium (GLA). Injection of GS inhibitors into the prepupal ES greatly reduced the glutamine content of the midgut epithelium by P;24. Although a corresponding increase in midgut glutamate levels was not seen, midgut serine levels in treated animals rose, suggesting that GS inhibitors shunted the MF-derived glutamate along an alternative metabolic pathway. GLA was much more toxic to pupae than MSO. Midgut GS appears to play a central role in the recycling of l-glutamate across the pupal MG epithelium at pupation.  2000 Elsevier Science Ltd. All rights reserved.
  • 【期刊】 Cellular distribution of a high-affinity glutamate transporter in the nervous system of the cabbage looper Trichoplusia ni.

    刊名:The Journal of experimental biology 作者:Richard B. Gardiner ; Kyrre Ullensvang ; Niels Christian Danbolt ; Stanley Caveney ; B. Cameron Donly 年份:未知
    摘要:Glutamate functions as a neurotransmitter in the central nervous system (CNS) and neuromuscular junctions in insects. High-affinity glutamate transporters are responsible for keeping the resting levels of excitatory amino acids below the synaptic activation threshold by removing them from the extracellular fluid, thereby preventing them from reaching toxic levels. Peptides representing the N- and C-terminal regions of a glutamate transporter cloned from the cabbage looper caterpillar (Trichoplusia ni) were synthesized and used to generate polyclonal antibodies. The antibodies produced immunohistochemical staining in both muscular and nervous system T. ni tissues. Neuromuscular junctions in the skeletal muscles produced the most intense labelling, but no visceral muscle or sensory nerves were labelled. In the CNS, the neuropile of the ganglia, but not the connectives, gave a diffuse staining. Electron microscopical examination of ganglia and neuromuscular junctions showed that the plasma membrane of glial cells, but not that of neurons was labelled, in agreement with the notion that most of the glutamate uptake sites in this insect are in glial cells.
  • 【期刊】 Rates of diffusion of fluorescent molecules via cell-to-cell membrane channels in a developing tissue.

    刊名:The Journal of cell biology 作者:R G Safranyos ; Stanley Caveney 关键词:Epidermis;Tracer;Membrane Channel;Diffusion Coefficient D 年份:未知
    摘要:Diffusion coefficients for the intercellular movement of fluorescent tracers have been measured in the epidermis of a larval beetle. Fluorescent tracer was injected into a cell and the spread of tracer from cell to cell in this monolayer was recorded by a TV camera. Fluorescence intensities were digitized from the TV images at successive times after the start of injection at various distances from the source by a microcomputer interfaced with a video analyzer. From the relationship between concentration (measured as light intensity), time and distance, an effective diffusion coefficient (De) is calculated for the tracer in the tissue. In newly ecdysed epidermis, De for carboxyfluorescein (CF) is 2.7 X 10(-7) cm2/s, and De for lissamine rhodamine B (LRB) is 1.2 X 10(-7) cm2/s, whereas in intermolt epidermis the De's for CF and LRB are 3.7 X 10(-7) and 1.2 X 10(-7) cm2/s, respectively. These diffusion coefficients are only an order of magnitude lower than their values in water. The ratio of De for the two tracers at these two stages of development differs from the ratio predicted in cytoplasm alone, with the movement of the slightly larger molecule (LRB) being impeded relative to that of the smaller molecule (CF). This suggests that the properties of the membrane channels amplify differences in the rates of movement of molecules of similar size. This may be important during cell patterning in development. De for CF was also monitored as junctional resistance was increased in the epidermis. During 30 min of exposure to 0.25 mM chlorpromazine, De dropped to 20% of its initial value of 5 X 10(-7) cm2/s, implying that the junctional membrane, rather than cytoplasm, is the major barrier to molecular diffusion among the cells.