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  • 【期刊】 Composition of the essential oils of Lycium barbarum and L. ruthenicum fruits

    摘要:Water-distilled essential oils from the fruits of Lycium barbarum and L. ruthenicum were analyzed by GCMS. The main components in the oil of L. barbarum were found to be hexadecanoic acid (47.5%), linoleic acid (9.1%), β-elemene (5.4%), myristic acid (4.2%), and ethyl hexadecanoate (4.0%). The essential oil of L. ruthenicum has heptacosane (14.3%), ethyl linoleate (10.0%), hexacosane (7.0%), nonacosane (6.2%), and ethyl hexadecanoate (5.8%) as the main compounds.
  • 【期刊】 Comparative analysis of carotenoid accumulation in two goji (Lycium barbarum L. and L. ruthenicum Murr.) fruits

    摘要:Background: The traditional Chinese medicinal plants Lycium barbarum L. and L. ruthenicum Murr. are valued for the abundance of bioactive carotenoids and anthocyanins in their fruits, respectively. However, the cellular and molecular mechanisms contributing to their species-specific bioactive profiles remain poorly understood. Results: In this study, the red fruit (RF) of L. barbarum was found to accumulate high levels of carotenoids (primarily zeaxanthin), while they were undetectable in the black fruit (BF) of L. ruthenicum. Cytological and gene transcriptional analyses revealed that the chromoplast differentiation that occurs in the chloroplast during fruit ripening only occurs in RF, indicating that the lack of chromoplast biogenesis in BF leads to no sink for carotenoid storage and the failure to synthesize carotenoids. Similar enzyme activities of phytoene synthase 1 (PSY1), chromoplast-specific lycopene beta-cyclase (CYC-B) and beta-carotene hydroxylase 2 (CRTR-B2) were observed in both L. ruthenicum and L. barbarum, suggesting that the undetectable carotenoid levels in BF were not due to the inactivation of carotenoid biosynthetic enzymes. The transcript levels of the carotenoid biosynthetic genes, particularly PSY1, phytoene desaturase (PDS), zeta-carotene desaturase (ZDS), CYC-B and CRTR-B2, were greatly increased during RF ripening, indicating increased zeaxanthin biosynthesis. Additionally, carotenoid cleavage dioxygenase 4 (CCD4) was expressed at much higher levels in BF than in RF, suggesting continuous carotenoid degradation in BF. Conclusions: The failure of the chromoplast development in BF causes low carotenoid biosynthesis levels and continuous carotenoid degradation, which ultimately leads to undetectable carotenoid levels in ripe BF. In contrast, the successful chromoplast biogenesis in RF furnishes the sink necessary for carotenoid storage. Based on this observation, the abundant zeaxanthin accumulation in RF is primarily determined via both the large carotenoid biosynthesis levels and the lack of carotenoid degradation, which are regulated at the transcriptional level.
  • 【期刊】 Virus-induced gene silencing in two novel functional plants, Lycium barbarum L. and Lycium ruthenicum Murr.

    摘要:Two species of Goji, Lycium barbarum Land Lycium ruthenicum Murr., are novel functional vegetables and functional fruits widely used in China and other Asian countries. Both species possess complex secondary metabolic pathways and show high tolerance and adaptability to saline-alkali stress, making them novel targets for functional genetic analysis of the biochemical pathways involved. Although stable transgenic Goji lines have been produced, the process is very labor-intensive and time-consuming. Virus-induced gene silencing (VIGS) presents an effective and rapid alternative for creating targeted gene knock-outs to study gene function in plants. In this study, the first application of VIGS in Lycium species is presented, using the Tobacco rattle virus (TRV) vectors. A number of vector delivery methods were trialed, including leaf syringe-infiltration, agrodrench, seedling vacuum-infiltration and sprout vacuum-infiltration (SVI). Vacuum-infiltration was the most effective method and was used to successfully silence two reporter genes, phytoene desaturase (PDS) and Mg-chelatase H subunit (Chi H), concomitant with photobleaching and yellow leaf phenotypes, respectively. The proven application of VIGS to these Lycium sp. will expedite the functional characterization of novel genes involved in the biosynthesis of functional components both in leaves and fruits, as well as the abiotic stress tolerances. (c) 2014 Elsevier B.V. All rights reserved.
  • 【期刊】 基于Illumina测序技术的枸杞微卫星引物的开发

    刊名:青海师范大学学报:自然科学版 作者:赵鑫[1] ; 李建民[1] ; 朱雪冰[1] ; 宗渊[3] ; 魏乐[1] ; 刘宝龙[2] 关键词:转录组分析 红果枸杞 黑果枸杞 微卫星位点 机构:青海师范大学生地学院 ; 青海师范大学生地学院 年份:2017
    摘要:目的:分离和鉴定红、黑枸杞基因组中的简单重复序列(SSR)位点.方法:Illumina Hiseq 2000进行红、黑枸杞的高通量转录组测序后,生物信息分析SSR的分布频率和重复基元的类型特征,PCR扩增和聚丙烯酰胺电泳验证SSR位点及引物.结果表明:转录组测序共得到了192,869条reads,总长度205,220,696bp.拼接后序列的数量为35,572条,序列平均长度约为1064bp.从中共预测到44,828个SSR位点,其中单、二、三、四、五、六个碱基重复单元的微卫星位点数分别有25,800、9186、8479、363、464和536个.从中随机选择30个位点,设计引物进行验证.23对引物在红黑枸杞中能扩增出产物,其中5对仅一个材料中有扩增产物,另有5个位点扩增产物能明显区分红黑枸杞.结论:利用Illumina测序技术得到的枸杞转录组中SSR位点出现频率高,类型丰富,对今后枸杞遗传多样性的研究以及种质资源鉴定打下了良好的理论基础.